Ka Ming Nip
Ka Ming Nip
@SaberHQ Good points! @kainth-amoldeep I suggest you can also try our development code on the master branch. We have had a number of bugfixes since v3.0.0.
Can you please report your version number and the exact command you used? How different is it from the expected number of reads?
Personally, I am not fond of the strategy of selecting random values within a while-true loop as it can definitely be a potential cause of an infinite loop. For this...
The main obstacle is to model the transcriptome heterogeneity within a population of cells. At the very least, there needs to be a model for the transcript expression profile for...
Hi @ad3002 , Instead of modifying the code of RNA-Bloom, you can work around the issue by simply giving the read names a "proper" prefix (e.g. "seq"). You can do...
Thanks for the suggestion, I have added a note about it in the readme.
I see that you started with 7.6 million reads in stage 3. You must have a lot of long reads as input! > Except their corresponding short reads under 25...
RNA-Bloom does not report that information.
Several questions: 1. Which version of RNA-Bloom did you use? 2. What was your command to fastp? I think fastp is more oriented towards short Illumina reads. 3. How many...
With R9, your direct RNA-seq reads would have a high error rate. With just half-million reads, there isn't a lot of error correction or polishing that can be done using...