Amoldeep Kainth

Hi, I am trying to use NanoSim 3.0.0. (downloaded source code from (https://github.com/bcgsc/NanoSim/releases/tag/v3.0.0)) for simulating ONT reads. I created my own profile using: `./read_analysis.py transcriptome -i ont_run.fq -rg ucsc_hg38.fasta -rt...

After re-estimating my transcripts using stringtiemerge with -eB option, I want to do differential expression analysis at the gene level. For that, I tried Ballgown as well as DESeq2 (after...