juliayork

The command was: ``` diamond blastp --query /path/ncor_cdhit.fasta.transdecoder_dir/longest_orfs.pep \ --db /data2/ref_seq_June2024/refseq_vertebrate_other.protein.dmnd --max-target-seqs 1 \ --outfmt 6 --out ncor_cdhit_td_blast ``` diamond version 2.1.9 let me know if you can reproduce the...

Good point. When I go back to recreate the error, it occurred only when I was running diamond through EnTAP. Running diamond alone on the files completes correctly. Seems to...

Hmm ok doing some more testing I was able to reproduce the error outside of EnTAP. The command is: `diamond blastx -p 8 -o /path/similarity_search/DIAMOND/blastx_ncor_cdhit_final_refseq_vertebrate_other.out --very-sensitive --max-target-seqs 3 --evalue 0.000010...

Great, thanks so much! Just for my reference, what's the argument for turning off the mutual coverage cutoff?

Perfect, thanks so much for all your help!