Junmin Liang

Hi Jon, I met the same problem when I run funannotate train with SRA reads download from NCBI. Open ERROR: FASTQ headers are not properly paired, see logfile and reformat...

Hi Jon, Thanks for your instant reply. Sorry I can not use gbk file to run antiSMASH since there are several alternative splicing for each gene and the error will...

Hi Jon, Could u give me an example for the signalp result used for fuannotate annotate?

I didn't know how to resolve it finally. I just remove --signalp from the funannotate annotate and try to merge the signalp results manually. Thanks.

Sorry，I may misunderstand your mean. Please see attachment to find the outputs of Signalp5 separately outside. The command I used for signalp5 was signalp -batch 30000 -org euk -format short...

> @zhichusun @caitiecollins You should check the version of snpEff, the error may happen with v5.1, and v5.0 is OK. I don't know why, but you can have a try....