jia-wu-feng

minimap2: align.c:125: mm_fix_cigar: Assertion `qoff == r->qe - r->qs && toff == r->re - r->rs' failed.

7

use -c for cigar, the error : minimap2: align.c:125: mm_fix_cigar: Assertion `qoff == r->qe - r->qs && toff == r->re - r->rs' failed.

Is it necessary for --anno 1 for pangenome in the initial annotation?

4

https://github.com/HuffordLab/NAM-genomes/tree/master/te-annotation Generate de novo TE library for each genome and perform initial annotation. Is it necessary for --anno 1 for this step?