John Cupitt

These are OME-TIFFs, right? I would make two one-band images and attach some XML metadata saying that the first is a stain and the second is a florescence image. You...

You need the `image-description` item. Try eg: ``` $ vipsheader -f image-description LuCa-7color_Scan1.ome.tiff

Right, but you need to set it to some XML to say what kind of images you have. Take a look at an OME-TIFF image that has the properties you...

You'll need to design some XML based on that, then set that as the image description of the TIFF you write. Eg.: ```python final_image = ... final_image.set_type(pyvips.GValue.gstr_type, "image-description", f""" ......

Hi @Borda, The `gap` param was added in 8.13 and the conda package hasn't updated yet. Are you sure you need to use gap? It's mostly an internal feature.

The python wrapper is (largely) generated at runtime, so the API you get is the API of the underlying libvips binary. Just drop in the `libvips.so` for 8.13 and you...

Oh, conda is a bit different, sadly, it lives in its own tiny mad world. You might have to wait for the conda package to update.

Hello @indridieinarsson, This is deliberate: tags like `grey16` say how pixel values should be interpreted, not what exact numeric format they are. `grey16` just means values in the range 0...

It's just the way globalbalance works. Images have to come from files that it can reopen, so they need to be tiff / vips / png sources, something like that.

You've not fixed your vignetting -- global balance just adjusts the overall brightness of tiles, it won't be able to adjust pixel by pixel. You could possibly take lens-cap black...