John Cupitt
Yes, the edge tiles will be 450x450, the centre tiles will be 500x500. `arrayjoin` only does exact grids. If you need to correct for motion errors, you can use the...
... a lot depends on how much control you have over the microscope. Can you adjust the camera pitch, yaw and roll, for example? Can you adjust the stage as...
Sorry, I don't have time to do the work for you. Find a local expert to help with the maths.
Hello, you've not flatfielded the frames. Post some sample data and I'll try for you.
Your tiles have not been flatfielded, something has gone wrong in that part of the process. Share your white image plus one tile of the slide, direct from the camera.
You need to take the white image using the microscope, using the same lens, focus and aperture as you use for scanning. I would do it as part of slide...
Your input image looks out of focus. It's sharp in the bottom middle, but very blurry at the edges. You will need to fix this as well. Perhaps you can...
You'll need to explain how you are merging them together. Post some demo code that illustrates the problem.
Sorry, I don't have time to code it up, maybe ask a maths friend? It's an over-determined linear system, so you just need to express the set of discovered overlaps...
Hi @xichenli00, It should work. Which libvips binary did you install? You need the `all` version for slide support: https://github.com/libvips/build-win64-mxe/releases/download/v8.14.2/vips-dev-w64-all-8.14.2.zip