Ida Shinder

The reads are allocated between the 5 transcript, based on read support. From the igv snapshot, I would guess that the reads are likely allocated between the 3rd and 4th...

Hi @MirkoBr - I am curious if you tried StringTie3 and whether it helped resolve the transcript ends (I believe it should have)?

@isaacvock - it looks like the coverage in your igv screenshot is from short reads? Is the assembly done with hybrid mode (with short and long reads)? When using short...

Hi @MirkoBr and @isaacvock, I believe that StringTie primarily focuses on reconstructing the correct intron chain and often collapses multiple termination sites into a single “representative” transcript that's in the...