Pay Giesselmann
Pay Giesselmann
same error here.
it happens, if you use the --rapid or --native flags, if you specify the models -s / -e manually, it works.
Hi @jts, I'm facing the same error as above using NGMLR v0.2.7 (-x ont --bam-fix) and nanopolish (v0.13.2 and latest build from master) It happened the first time and is...
Awesome, that fixed it!
Hi, sorry for the late response: I don't have a barcoded dataset that I can make public available. The demux funciton of nanopype is based on guppy_barcoder, so results should...
Ah I see, I meant code from nanopype, in the repo in rules/demux.smk you find the usage of guppy_barcoder. You mean demultiplexing and not barcoding, right? I'm not sure if...
Hi, yes, you can alter the install.smk and generate your own singularity based on that. Under test/build_singularity.sh you find the docker commands for the build (I'm only familiar with docker...
Hi, The repeat count 0 is given for reads where for instance the signal alignment of prefix and suffix failed. These reads can be filtered out, I updated our documentation...
Hi, There's no development from our side regarding R10 support at the moment. ONT ist no longer providing kmer models (https://github.com/nanoporetech/kmer_models), one would therefore need to derive one from R10...
I would check how good basecalling has become and try a sequence-based classification, either RepeatHMM or a simple decoy alignment. With R9, we've seen strand-specific errors, if you plot R10...