Jan Eglinger

The multi-point tool is an [`imagej1`](https://github.com/imagej/imagej1) feature. /cc @rasband

Sure, you can use this Groovy script for example: ```groovy #@ ImagePlus imp roi = imp.getRoi() poly = roi.getPolygon() println poly.xpoints println poly.ypoints ```

@chalkie666 > Note that the 3D ROI manager available as an update site does 3D object coloc, so we dont need to re do that immediately at all!!! Note that...

Duplicate of https://github.com/imagej/imagej/issues/151 Can you explain more clearly what's the problem, please? See http://imagej.net/Bug_reporting_best_practices

Seeing the discussions in #474 and #475, is the plan still to have first-class support for Pydantic classes? Or should I rather look into using `@dataclass` or `@guiclass`?

> Can you read through this topic on imagesc and let me know what your specific desired use case would be? > [forum.image.sc/t/building-a-napari-widget-from-a-pydantic-model/90257/5](https://forum.image.sc/t/building-a-napari-widget-from-a-pydantic-model/90257/5) Thanks @tlambert03, that's a very useful forum...

## Story 22: Registration of multiplexed imaging Goal: creating multi-channel (3d or 2d) image from multiple rounds of staining/acquisition. 1. Acquire multiple rounds of 3-channel images with various markers, e.g.:...

@leonart3 how did you try to open the LSM file? Via drag-and-drop? Or with *Plugins > Bio-Formats > Bio-Formats Importer*? Also, could you please check whether *Use SCIFIO when opening...

You should convert to *unsigned* 8-bit when converting with ops, no? ``` converted = ops.convert().uint8(inputData) ```

Ah yes, that's because `convert` doesn't scale the intensity values at all, and high values simply "wrap around" the 8-bit limit.