hannan666666
hannan666666
Thank you very much for your kind and informative reply! If possible, could you share the species and the 4mC fraction of your validation sample? My sample is from a...
Thank you for your response. Yes, ${modkit_d}/${sample}.pileup.bed.gz is a bgzip-compressed bedMethyl file. The uncompressed file is approximately 100GB, and the reference sequence size is 2.6GB. My machine has 1.5TB of...
Thank you for your nice reply! This is the rough "overall modification" level in my sample: (base) hannan@RS720A-E12-RS12:/data3/hannan/data/nanopore_process/nanopore_04_modkit/WT_test$ cat WT_test.6mA_5mC5hmC.summary # bases A,C # total_reads_used 10042 # count_reads_C 10028 #...
Well received, thank you for your advice!
Hi, @ArtRand sorry to bother you — I’d really appreciate your help or suggestions.
> [@hannan666666](https://github.com/hannan666666) > > Sorry for the delay. No worries at all—whenever you have time! Looking forward to your reply !
Hello, @ArtRand This is absolutely great — thank you for the detailed and helpful reply! Your suggestions are very valuable and exactly what I needed. Much appreciated! Best regards!