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Transcript assembly and quantification for RNA-Seq

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[An extra null pointer check is not needed in functions](https://isocpp.org/wiki/faq/freestore-mgmt#delete-handles-null "Do I need to check for null before delete p?") like the following. - [basic_circular_buffer](https://github.com/gpertea/stringtie/blob/b4366c542af25f9f25e37c434858718d2f757b3c/SuperReads_RNA/global-1/jellyfish/include/jellyfish/circular_buffer.hpp#L59-L62 "Destructor for basic_circular_buffer") - [Value](https://github.com/gpertea/stringtie/blob/b4366c542af25f9f25e37c434858718d2f757b3c/SuperReads_RNA/global-1/jellyfish/lib/jsoncpp.cpp#L2035-L2036...

Dear Geo, I had different assembled transcriptomes depending by the usage of STAR or HISAT2 with the latest version of stringtie. You can see a screenshot of a region I...

Hello, I’m using StringTie to process my Direct RNA Sequencing (DRS) data, and I have a few questions regarding the usage of certain parameters. Below is the command I used:...

Hello When I run --conservative mode I get very different results to when I run -t -c 1.5 -f 0.05 even though the manual says its the same. eg. my...

I'm trying to use stringtie to quantify lncRNA data, the lncRNA gtf file is generated from stringtie. I find that the expression matrix is different from the matrix generated by...

I am running the latest release (2.2.3) on an ONT dataset that is mapped to GRCm39 using minimap2. stringtie halts at the following bundle regardless how much memory space I...

to investigate

If I use `--fr` or `--rf` to specify the library strandedness, my understanding is that I can use a BAM without XS tags. Am I right?

Hi StringTie team, I have been looking for answers in different forums and then thought let me write here in the hope of an answer to my simple question, which...

Hi, When I assemble transcripts using StringTie2 (-mix), the process is terminated by signal 11. I noticed that more than 70% of the reads are aligned to chromosome 6 (in...

Hi, I've been doing RNAseq analyses with the HISAT/Stringtie pipeline for the last few years. Our computing centre updated their hardware a few weeks ago and subsequently uninstalled some older...