Nameless_Wonder

> Hi, Thanks for your reply. 450 px was typo sorry. I ran with a particle box size of 256 actually during extraction. I am trying with 128 now. 2D...

_How did you make it? I suspect you made some mistakes here._ I made it using python scripts written by Dustin Morado (https://github.com/DustinMorado/EPU_group_AFIS). People from where we collected this data...

_But the fact that the same STAR file worked fine in RELION 3.1 suggests something else..._ I didn't save the non-dose weighted on Relion/3. Also, how important is it to...

> Good. Don't save in RELION 4, too Thanks, I tried. But that error persists. These were my corresponding job commands. Please advise if I'm doing anything wrong? Select: `which...