dpaudel-tb

Unexpected base in duplex call

4

Hello, I extracted fastq from the duplex_orig.sam and compared output to the original raw reads. In the following alignment file, The top sequence is the output for the duplex read....

Extracting duplex reads for multiplexed samples

2

Since barcoding is still under development in darado, I was wondering what alternative approach would be a proper way to extract duplex reads for multiplexed samples? I am thinking along...

Differing read count number in simplex and duplex basecalling

2

Hello, I ran simplex and duplex basecalling on the same dataset (dorado-0.4.1-linux-x64 with [email protected]). I was expecting to get same number of reads on the simplex basecalling and the duplex...