dithiii

I have the same question. I have recently used megalodon to basecall mammalian mitochondrial reads from a 9.4.1 minion run. Most were at 0%, as expected, but overall, the average...

Thanks for the detailed response. I used megalodon rather than guppy because I already had an assembled genome that I wanted to get methylation calls for, rather than calling with...

Just an update. I re-ran the basecaller with guppy, using the old research model with >80% stringency, `res_dna_r941_min_modbases_5mC_5hmC_v001` and got: 0.2% 5hmC 0.13% 5mC Then I ran the updated model,...

Hi @kaanokay , Yes I have dealt with that issue in the following way. Concatenate all the unmapped bam files containing modified base information. `samtools cat -o mod_basecalls.bam *input.bam` Map...