Quang Nguyen

@Gpasquini -- Would you mind writing out the full command to find the right directory and then to access velocyto from it? Thank you so much!

@saeedfc @shabs24 After you cellsort the possort bam file, did you just use the same bam.bai index file that came with the possort bam from cellranger? Or, did you index...

@RBBurl1227 your ./sampledirectory is the directory contains the "outs" folder, or the outs directory which contains the bam file (i.e. /outs )? From the tutorial, it says it should be...

@kumtri - the short answer is you should run it on the cluster.

@shaaaarpy -- Did you resolve this memory issue?

Thank you so much @f6v for your input. The 3 donor datasets for CD39neg were sequenced together. The 3 donor datasets for CD39pos were then later sequenced. (1) When I...

@UboCA @rockdeme and @AmirAlavi -- the `t` in the `CreateSeuratObject()`? This step threw an error saying my adata$X is not a matrix. Do I need to convert the dgRMatrix to...

@ghar1821 - Did you mean in Python, you downgraded anndata to 0.7.6, then do `adata.T.T.write_h5ad` before using `Convert` and `LoadH5Seurat` in R? I did that, and in R, when I...

hi @pinunQ - `peri = LoadH5Seurat("~/peri.h5seurat", meta.data = FALSE, misc = FALSE)` still gave the same error. Do you know why?

When unzip the `barcodes.tsv.gz` into `barcodes.tsv`, it works, but encounter error mentioned in #321