Dario Copetti
Dario Copetti
hello, I wonder if it is possible estimate the heterozygosity present in my sequencing reads from a distribution like this:  It is from raw Illumina reads of a heterozygous...
Hello, I am scaffolding a Hifiasm assembly of a selfed tetraploid plant (~400 Mb assembly, contig N50 11.7 Mb, N90 2.5 Mb) with a reference assembly created by merging the...
Hello, I am running MaSuRCA on a plant genome, I have PacBio and Illumina data as input. I just noticed this notification: ``` $ ./assemble.sh [Mon Sep 21 16:13:10 CEST...
Hello, I would like to align PacBio HiFi reads to ONT reads with the goal of making a consensus of the alignment against the long read backbone (to correct the...
Hello, I have a problem similar to [issue 222](https://github.com/chhylp123/hifiasm/issues/222), but actually it is the opposite. I am assembling a plant genome from two SMRT cells of HiFi data. The k-mer...
Hello, I wonder if there is a way to choose right away the best `-l` value when working with genomes at different levels of heterozygosity. The genome I am handling...
Hello, I am assembling a highly heterozygous plant genome (haploid genome of ~2.6 Gb) from about 42x HiFi coverage (21x per allele). My priority is to have phased sequences to...
Hello, I am dealing with a complex plant genome (higly heterozygous, haploid genome size 2.5 Gb, I have a diploid assembly of 5 Gb, N50 200 kb, N90 5 kb)...
Hello, I am running Falcon on a ~800 Mb genome with ~60x coverage. It keeps dying at the step 1 with the following error: ``` ... [INFO]'/gsfs1/rsgrps/rwing/tania/falcon_90_subset/1-preads_ovl/job_0d47/job_0d47_done.exit' found. [INFO]_refreshTargets() finished...
hello, I am preparing the inputs for MCScanX and I find this incongruence: you say you want a .bed file, but the format you give in the readme is different:...