Rubens Daniel Miserani Magalhães
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Rubens Daniel Miserani Magalhães
I forgot to mention the GRCh38_basic.gtf and GRCh38.fa was downloaded from gencode. Gtf. Minimap2 was used to alignment: minimap2 -ax splice --MD -k14 -uf --secondary=no -t 20 path_ont_fastq