Chris Fields

Not a pressing issue. This may be one we can possibly add some basic materials for to start it off.

Coming into this a bit late, but there is a random seed setting as one of the parameters and sampling is mentioned in the documentation, so I think this is...

> Dear @cjfields, > > Could you clarify how you use the `-r` option and with which version of the tool? Also, I do not see this option listed when...

I'll give it a try, thanks @benjjneb !

This installed without issue, thanks @benjjneb

@nathanjohns see some answers below, though I'm curious what Ben says. > > drp > Dereplicating sequence entries in Fastq file: 20231010_rRNA.demux.0--0.trimfiltmerge.fastq.gz > > Encountered 93299 unique sequences from 454842...

We've been using something like this for trimming using `cutadapt`: ``` # revprimer_rc is revcomp of reverse primer cutadapt --rc \ -g "${fwdprimer}...${revprimer_rc}" \ -j ${cores} \ --untrimmed-output "${id}.untrimmed.fastq.gz" \...

--- Original Redmine Comment Author Name: **Charles Plessy** Original Date: 2011-06-18T02:11:01Z --- I forgot to list Tree::DAG_Node as well.

--- Original Redmine Comment Author Name: **Chris Fields** Original Date: 2011-07-06T02:29:21Z --- The traditional place to have these is in a `build_requires` section. Would that work?

--- Original Redmine Comment Author Name: **J Nelson** Original Date: 2012-02-02T16:40:24Z --- Update: The patch I originally submitted did not work on Win32 machines, due to File::Spec->catfile() turning the ‘/’...