Chris Fields

> > Also, have you had a chance to check the monotonicity enforcement against the data sent by @cjfields? > > I haven't yet got any NovaSeq test datasets from...

@benjjneb I didn't have much luck in getting NovaSeq Zymo data, but I did a search and there is [one NovaSeq Zymo control sample](https://www.ncbi.nlm.nih.gov/biosample/14109336) in SRA in [project PRJEB36316](https://www.ncbi.nlm.nih.gov/bioproject/PRJEB36316). No...

> This is coming up enough that we ought to at least implement an enforced monotonicity option in the main package. > > We are still waiting for good test...

I should add, the log file is very small (as you can see above). I may test copying the database to local disk on the worker node and running it...

Hi @aaronmussig we were able to work around this by being very selective with the bins we used. I suspect the issue is as @pchaumeil outlined.

@johnne interesting result! I'm guessing this is with the BOLD database? We had issues with this one and used a VSEARCH-based approach, but now this makes me wonder if we...

Looking at the table, I think there might be issues with how samples are being tracked in your custom pipeline and issues with the pipeline logic, possibly between the filtering...

@JacobRPrice really impressive work! Would be great to get @benjjneb thoughts, though this argues that one could at least try different approaches with some follow-up QA to see what works...

> We're trying to develop a lab pipeline and seeing as it looks like we'll be dealing with NovaSeq data going forward, my current recommendation for users of NovaSeq data...

@benjjneb I do have some code that does this, implemented as a workaround in our workflow code (specifically for ITS and other variable length sequences). If I could get it...