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Fast taxonomic classification of metagenomic sequencing reads using a protein reference database

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I am trying to taxonomically classify my reads using only the viral database. This is the code I used: kaiju -t /home/akhwela/kaijudb/viruses/nodes.dmp -f home/akhwela/kaijudb/viruses/kaiju_db_viruses.fmi \ -i home/akhwela/lustre/Data/noSheepQCRead/*.fastq.gz I keep getting...

I would like to thank you so much for this wonderful tool. I really like it so much. However, I would appreciate if there is a way to reduce memory...

Is there any way to convert the kaiju output to the biom format? Short of writing my own code to do it?

Hi Abigail, For some reason eukdetect gets stuck at the taxonomize stage, i.e., snakemake sleeps and is forever stuck. I thought it was an error with ete3, and even regenerated...

So I have created a custom database by taking the refseq proteins and adding proteins from a database called RUG2. When I run classification with the regular refseq database on...

Hello, thank you for creating this amazing tool! I'm trying to custom a uniref90 DB from the uniref90.fsa file. I have made the file has a format like >1_1572043 MEEITQIKKRLSQTVRLEGKEDLLSKKDSITNLKTEEHVSVKKMVISEPKPEKKEDIQLK...

Dear Developer, Thank you so much for Kaiju. I performed spade assembly (for the virus) and performed kaiju on assembled contigs. For the reference database, I downloaded the viral database...

Downloading the nr_euk database using the kaiju-makedb is failing with the following error: Extracting taxdump.tar.gz/... gzip: stdin: invalid compressed data--format violated tar: Unexpected EOF in archive tar: Unexpected EOF in...

Hi, I have a question about the Kaiju output. I want to extract the AA sequences derived from fungi from each sample. My goal is to use the sequences as...