biofer

How to use the location of 2-4bp as UMI of reads at 5' ?

2

Thanks for your great work。 I want to remove the begin 1 bp and use the 2-4 bp as UMI , did `fastp` support this? ``` fastp ... \ --trim_front1...

🐛🐛 Bug Report: 机器人频繁自动掉线

1

## 之前都好好的，昨晚上开始机器人就开始频繁掉线！！！ > 也没有什么报错，不知道怎么调试。 @wj-Mcat ## Error info * 2021-08-19 11:32:29,697 - HostiePuppet - DEBUG - receive message info 2021-08-19 11:32:29,697 - Wechaty - DEBUG - receive event 2021-08-19 11:32:29,697...

🐛🐛 Bug Report: 群里拍一拍，不能捕获具体谁操作的

1

## 群里拍一拍，不能捕获具体谁操作的 > 在群里拍一拍，返回历史记录是下面。没有返回具体谁操作的。 ## Reproduce your problem ```python async def on_message(self, msg: Message): from_contact = msg.talker() text = msg.text() room = msg.room() if msg.type() == MessageType.MESSAGE_TYPE_RECALLED: print(from_contact) ``` ##...

https://github.com/wechaty/python-wechaty-getting-started/blob/209511c1dfa6dbb29407192b51d7aa9c0260de5d/examples/advanced/friendship-bot.py#L35-L37  The real other people id? Why not use the user's own ID. ```python async def on_friendship(self, friendship: Friendship): administrator = bot.Contact.load(self.contact_id) await administrator.ready() ```

How to use `mutscan` with UMI ?

1

when use `fastp` + `gencore` , can not use `GATK SamToFastq`. `gencore` copy the flag of R1 reads as R2 reads (or R2 as R1)? For example， Before `gencore` ```...

### Is there an existing issue for this? - [X] I have searched the existing issues and checked the recent builds/commits ### What happened? I ran the code as per...

Donut: 确定6个月后不能用了么？那有其他替代方案么？担心开发代码后不能用了。

3

Please make sure you have read the [Wechaty Puppet Service: Donut](https://wechaty.js.org/docs/puppet-services/donut/) from Wechaty Official Website before you continue writting this issue. ## Question

When setting ETL_TYPE to Unstructured, the local docker runs and reports an error when creating the knowledge base.

7

### Self Checks - [X] This is only for bug report, if you would like to ask a question, please head to [Discussions](https://github.com/langgenius/dify/discussions/categories/general). - [X] I have searched for existing...

As per the previous response, I have modified the docker-compose.yml file to map the host directory to the container's /root/.ragflow directory to avoid re-downloading models on container restart. My updated...