Andrea Martinez Vernon

Hi Kohei, You have to first annotate the metagenomes with KEGG orthologs (K numbers). PICRUSt could be a useful tool for that. Have a look at this tutorial: http://picrust.github.io/picrust/tutorials/metagenome_prediction.html#metagenome-prediction-tutorial Once...

Hi, You do seem to need the 16S sequence (sorry, I have no experience annotating metagenomes). [This tutorial](http://www.edamamecourse.org/docs/metagenome_assembly_and_annotation.html) could walk you through using [prokka](http://www.vicbioinformatics.com/software.prokka.shtml) to annotate the metagenome sequence. Once...

Hi again, Just came across this paper that could also be useful to you: https://genomebiology.biomedcentral.com/articles/10.1186/s13059-016-1116-8 :)

Hi Abraham, Could you provide more info about the analysis you want to do? What is the expected outcome or the question you're trying to answer? All the best, Andrea...