Arun Seetharam

Thanks for bringing this to our attention. We will keep this issue open until the [official Trinotate](https://github.com/Trinotate/Trinotate.github.io/issues/9) comes up with suitable solution. We are simply using their recommended steps and...

@EdderDaniel, we have not tested it on RSII, but yes, it does need BAM file as input. If you want to try, please be sure to use subreads. Thanks,

Sorry, I've not dealt with bax.h5 files for a while now, so not sure what works. However, a quick Google search brought this result up, which looks promising. https://github.com/EichlerLab/bax2bam Hope...

@J-Calvelo: Thanks for trying SequelTools! From the error message, it seems like you are not using the actual scrap file (the parsed tags were missing in the file, I think?)....

@senzei-21 It looks like you are running SequelTools on CCS/HiFi reads - unfortunately, this tool only works on subreads. Please try this on your subreads and let us know if...

Yes, @asantat, that should work. When you download the file using tools like `wget` or `curl`, it automatically renames the files with the `.1` extension, if there is already a...

Hi @JiaweiShen1116! From the message, it looks like your bam file is truncated (or corrupted). Can you check the last line of your bam file to see it has all...

@JiaweiShen1116: are you sure you are using the subreads bam file as produced by the sequel? If you have converted the fastq files to bam, SequelTools will not work as...