Input BAM file not found
Hi,
I am trying to run lumpyexpress but I am getting the following error despite the bam file exists and the path is correct:
(base) elpz@picasso:/mnt/scratch/users/b02_cimes_uma/elpz/SVs_manta_normal/021LH19/manta_normal/manta_results> lumpyexpress -B /mnt2/fscratch/users/b02_cimes_uma/elpz/lhodgkin_try3/021LH19/results/8_applybqsr/0/021LH19/0_021LH19.bam Sourcing executables from /usr/local/bin/lumpyexpress.config ...
Checking for required python modules (python)...
usage: lumpyexpress [options]
options: -B FILE full BAM or CRAM file(s) (comma separated) (required) -S FILE split reads BAM file(s) (comma separated) -D FILE discordant reads BAM files(s) (comma separated) -R FILE indexed reference genome fasta file (recommended for CRAMs)
-d FILE bedpe file of depths (comma separated and prefixed by sample:) e.g sample_x:/path/to/sample_x.bedpe,sample_y:/path/to/sample_y.bedpe -o FILE output file [fullBam.bam.vcf] -x FILE BED file to exclude -P output probability curves for each variant -m INT minimum sample weight for a call [4] -r FLOAT trim threshold [0] -T DIR temp directory [./output_prefix.XXXXXXXXXXXX] -k keep temporary files -K FILE path to lumpyexpress.config file (default: same directory as lumpyexpress) -v verbose -h show this messageError: file /mnt2/fscratch/users/b02_cimes_uma/elpz/lhodgkin_try3/021LH19/results/8_applybqsr/0/021LH19/0_021LH19.bam not found.
real 0m0,492s user 0m0,263s sys 0m0,188s
How may I fix this?
Thank you in advance!
Cheers,
Espe