Missing pair end parameters:bamfile, histo_file, mean, stdev, read_length, min_non_overlap, discordant_z

[psardana22]

Hi lumpy team, I have been working on running lumpy to call large structural variants. I have been running lumpy traditional where I generated insert size distributions following the below command. samtools view -r readgroup1 sample.bam
| tail -n+100000
| scripts/pairend_distro.py
-r 101
-X 4
-N 10000
-o sample.lib1.histo However, after this when I try running the lumpy , it shows me missing pair end parameters even after mentioning the parameters. Could you please help? I am attaching the snippet of the code that I have written. Looking forward to hearing from you

Thanks and regards, Prachi