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What does -5 mean in bedtools genomecov?

Open Rhine86 opened this issue 5 years ago • 2 comments

help message shows that -5 mean "calculate coverage of 5' positions instead of entire interval", but I don't know what's 5' position exactly means?

ZHANG Yan

Rhine86 avatar Sep 28 '18 04:09 Rhine86

Thanks for this question! I am wondering exactly the same thing at the moment. Did you find an answer to it? I look into my bam file with tview, but the numbers genomecov -5 reports for certain positions doesn't make perfect sense to me, I can't see any pattern about what it exactly counts..

FatihSarigol avatar Jan 14 '19 16:01 FatihSarigol

I'm curious to know how this setting treats soft clipping -- is it 5' of the alignment, or the query?

cmatKhan avatar Oct 01 '22 17:10 cmatKhan