Ran out of memory while applying -64982367872bytes

[WJT0925]

#################### my config ########################### max_rd_len=150 [LIB] avg_ins=350 reverse_seq=0 asm_flags=3 rd_len_cutoff=150 rank=1 pair_num_cutoff=3 map_len=32 q1=~/1/Pdong_data/Filter_bac/L7_wHAXPI020290-35_unmap_rd_1.fq.gz q2=~/1/Pdong_data/Filter_bac/L7_wHAXPI020290-35_unmap_rd_2.fq.gz q=~/1/Pdong_data/Filter_bac/L7_wHAXPI020290-35_unmap.single.rd.fq.gz q1=~/1/Pdong_data/Filter_bac/L6_rd_1.fq.gz q2=~/1/Pdong_data/Filter_bac/L6_rd_2.fq.gz q=~/1/Pdong_data/Filter_bac/L6.single.rd.fq.gz ##################### my cmd ###############################
#!/bin/bash #SBATCH --nodes=3 #SBATCH --ntasks-per-node=56 #SBATCH --partition=D2Part_test #SBATCH --job-name=sopadenovo_test #SBATCH --output=sopadenovo.29.out #SBATCH --error=sopadenovo.29.err ~/software/SOAPdenovo2-master/SOAPdenovo-63mer pregraph -s config_file -o PB_29 -R -K 29 -p 168 > PB_29_pre.log ~/software/SOAPdenovo2-master/SOAPdenovo-63mer contig -g PB_29 -R -p 168 > PB_29_con.log ~/software/SOAPdenovo2-master/SOAPdenovo-63mer map -s config_file -g PB_29 -p 168 -k 29 > PB_29_map.log ~/software/SOAPdenovo2-master/SOAPdenovo-63mer scaff -g PB_29 -F -p 168 > PB_29scaf.log date ####################### log ################################# 206 ******************** 207 Contig 208 ******************** 209 210 Parameters: contig -g PB_29 -R -p 168 211 212 There are 566067075 kmer(s) in vertex file. 213 There are 1830549853 edge(s) in edge file. 214 Ran out of memory while applying -64982367872bytes 215 There may be errors as follows: 216 1) Not enough memory. 217 2) The ARRAY may be overrode. 218 3) The wild pointers. 219 220 Version 2.04: released on July 13th, 2012 221 Compile Dec 31 2021 12:53:17 ############################################################# This was used K=29, and forward and reverse reads total 400GB, predict Genome size 4.2Gb, and jellyfish analysis k = 28. My linux server memory = 1.5TB, I also used K=127 with no error came out. So can you help me about what's wrong with k=29 ? Thanks !