Andreas Wallberg

I am bitten by this issue too. I can not index my 18Gbp genome assembly on a 512GB RAM node.

Did you resolve this issue @Shians or come up with an enhanced workflow? I am in a similar position. We have over 80 million PromethION reads and around 10TB of...

Thanks for the feedback! I fully appreciate the complexity and time needed to add new functions to old but working code :-) With "handle multiple accessions as is", I mean...

Makes a lot of sense! I set up a pipeline for dN/dS in PAML using high-quality pairwise alignments a few years ago. Perl is my main language, so perhaps I...

Hi @krabapple! I am interested in the same problem. Did you solve it?

Dear @jdemeul did you manage to find a solution? We are seeing coverage issues in training step resulting in few accepted reads used to build the model, and later on,...

Hi @jdemeul , thanks for the feedback! Much appreciated. Could you please indicate to values you changed those parameters? Could you link to (or send a pm or email, I...

Dear @HanielF and @iiSeymour We appear to be in a similar position. Several millions of reads as input, but only tens of thousands of reads appear to be used. My...

Great answer. Many thanks! In the case of a longer haplotype block, would you recommend to average estimates across multiple focal variants, or expect the method to return similar estimates...