amit4mchiba

Thank you so much for the reply. I checked the files, and it had all the files. So, I still do not know why the error. But one thing that...

Thank you so much for such a quick reply. Actually I got HiFi sequencing for same sample but through different providers and at different time points. And the reads that...

Thank you so much for your reply. Following your advice, I firstly used subreads and performed the extraction of Hifi reads by firstly running ccs and then extracthifi for all...

Goodmorning from Japan. I started re-running HiFIASM, and although it is not over yet, the first kmer plot seems like this (command used were-hifiasm -o Nfoetida_hifi_vaishu3.asm -s 0.5 -t48 -f39...

Dear Kinggerm, Thank you so much for your reply. I tried following your suggestion and used following command- get_organelle_from_reads.py \ -1 /mnt/HD1/Sophla_HiC_juicer/Plant_genome_related/Sf_ill_paired_R1.fq.gz \ -2 /mnt/HD1/Sophla_HiC_juicer/Plant_genome_related/Sf_ill_paired_R2.fq.gz \ -s ./Sf_mito_seeds1.fasta \ -a...

Thank you so much for the advice. I am running what you suggested and will post you once result is ready. Thank you so much.

Thank you so much for your reply. I followed ur instructions, and got following errors- File "NanoFilt.py", line 41 if args.length

I am so sorry for another question. In the readme file, one suggested quick run command is - gunzip -c reads.fastq.gz | NanoFilt -q 10 | gzip > highQuality-reads.fastq.gz I...

Thank you so much for your reply and I am so sorry to keep asking so many questions. I used the edited version of python script and this is the...

Thank you so much. There were still very minor error but I was able to solve it. The error was due to the last few lines, and the script that...