[results]

[JensFGG]

I ran the complete pySCENIC pipeline and ranked the importance of the regulons with the RSS function per subtype of macrophages (roughly 12 different subtypes).

It occurs often, however, that the most important transcription factor has hardly any expression in that specific cluster. Does it take the activity of the regulons - so the target genes - into account when calculating the RSS? Since lack of expression with scRNA does of course not mean that it is not present due to the relative superficial sequencing.