ZeyanZhang

Hello @Hoohm , Thanks so much! I do see a lot of hits by grep, and they are in the middle and followed by poly As, please see below of...

Thanks @Hoohm , I adjusted as your suggestion below, and I changed -umil from 26 to 28 because the R1 length is 28 and 10x v3 CB+UMI is 28. The...

And if I grep one tag on logouts, I do see best matches. But it's still strange that the result is still 100% unmapped. Best match is: EGFR-GCTTAACATTGGCAC line:TCATGCCTCGAGAGACGGTTAGAGGACCAAGCAGTGGTATCAACGCAGAGTACATGGGGCACCCGAGAATTCCAGCTTAACATTGGCACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA cell_barcode:TCATGCCTCGAGAGAC...