Liu Yang

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Thank you for your suggestion! But the fisrt step I have tried is --fast5 and --fastq. Disappointingly, this step did not work . My fast5 file has no Analyses Group...

Hello, My goal is to measure the polyA length of cdna in large quantities using tailfindr or PolyACaller with SQK-PCS111, both of which require a trace table. Rebasecalling with stand-alone...

And for large genomes, memory usage is also very scary, the peak memory usage of `modkit pileup` reached 150G, and `modkit find-motifs` has exploded a server with 500GB of memory....

I have read the documention of Performance Consideration;but it has almost no effect on my doubts. For 300GB input files, the memory has already exploded before seed searching step

I have read the documention of Performance Consideration;but it has almost no effect on my doubts. For 300GB input files, the memory has already exploded before seed searching step

I have read the documention of Performance Consideration;but it has almost no effect on my doubts. For 300GB input files, the memory has already exploded before seed searching step

my modkit version : ![image](https://github.com/user-attachments/assets/72e996d3-d28d-4acc-85bf-c1151598d82d)

I found the problem. The problem is that the output folder must exist, otherwise an error will occur after creating the folder.

The reason I found seems inaccurate because I encountered this error again. Looking forward to software updates