Wshengquan

Hi I am running guppy_basecaller 4.4.2(CPU mode) for the basecalling. My running command: guppy_basecaller --input_path fast5 --save_path output_fast5 -c dna_r9.4.1_450bps_hac.cfg --qscore_filtering --cpu_threads_per_caller The log message is below: 2923-09-21009:15:55.857479 [auppv/message] ONTGuppy...

KeyError: ('C', 1, 'm')

32

hello， I got an error when I used mathphaser： /share/home/yzwl_hanxs/anaconda3/envs/danbeixing/bin/meth_phaser_parallel:235: FutureWarning: When grouping with a length-1 list-like, you will need to pass a length-1 tuple to get_group in a future...

No methylation information was calculated for some reads

3

Hi，I used this command to extract methylation information： modkit pileup ${mod_bam} ${bedmethyl_cpg} --cpg --ref ${ref} --no-filtering However, I found that the CpG methylation information in the result file did not...