ThomePauline

Using --revcomb on paired-end data

1

Hello, I have a few questions and observations about the behaviour of the --revcomb flag for when cutting off primers from paired-end data. 1. Using a single-read approach on F...

Hi @benjjneb , after losing >60% of reads (43M total, Illumina PE 2x300 bp, 18S V4) through filterAndTrim(..., maxEE = 2, truncQ = 2, maxN = 0), we tried two...