Taylorain
Taylorain
Hi George, I encountered issues while using Dorado for basecalling. Two different POD5 files have thrown zstd-related decompression errors, and eventually resulted in a segmentation fault. Below are the key...
I’m not entirely sure — could you please advise on how to check the integrity of the pod5 files? I’d like to verify whether they have indeed become corrupted during...
Thank you pretty much!
Hi, I'm also using modkit for DRS analysis, but I'm unsure about how to determine the filter threshold and --mod-thresholds for different base modifications (m6A, m5C, pseU). Is this the...
@ArtRand I get, thanks pretty much!
Sorry, my previous description might not have been very clear. 1. When using the `[email protected]_inosine_m6A@v1` model, I observed that both inosine (I) and m6A modifications were detected at the same...
``` samtools view -@ 80 -q 1 -b 0_A.bam | samtools sort -@ 80 -o 0_A.sorted.bam samtools view -@ 80 -q 1 -b 0_A.inosine.bam | samtools sort -@ 80 -o...
I have checked these two BAM files and confirmed that the read alignment information is identical. - **Extracting basic alignment information:** ```bash 0_A.bam | awk '{print $1, $3, $4, $6}'...
For RNA data in a SAM file, can I interpret "m" and "a" as representing m5C and m6A modifications, respectively? For example, in the tag `MM:z:A+a?,5,5,4`, can I consider this...