Siyi Wanggou

I meet the same error. I am just wondering what's the meaning of "-t CB" in samtools sort here? Could you show me about this? Thanks,

Does CB meanings "Cell Barcodes" here?

@kaizen89 Thanks. I get some information from 10X genomics website. It discibes CB as "Chromium cellular barcode sequence that is error-corrected and confirmed against a list of known-good barcode sequences"....

@yanwengong Are you using the data from 10X Chromium samples? The filtered_gene_bc_matrices folder seems to be the results of cellranger. If so, you should follow the instruction for 10X.