Rob Murphy

That makes sense. Thank you :) What if one is not to sure what should be considered expected in that environment given it has been sparsely sequenced in the past?

Not meaning to necro this but it only seems to occur when using ´by="margin"` with interactions specified in the formula. For example: ``` # This will give the error >...

Apologies I was not trying to seek help just state my experience. I can give data for a reproducible example tomorrow but for what it is worth it seems to...

Okay thank you :) What should I do for samples when I had to pool PCRs? (A stupid question I know but I can't find a solid answer on how...

I measured before pooling but when pooling I did not always take the same volume. For example it may have been 40 ul of one and 8 ul of another...

An example command from the bash script would be ``` cd /home/lamma/local-blast/termitomycesBGI/Genome_v3/termitodb # to get me into the database directory as it didn't seem to work if I was not...

hi, where should I upload the sequence files to?

Hi there, sorry for the slow reply. I have attached an example sequence file. [P1_19_mod.zip](https://github.com/mcfrith/last-genome-alignments/files/5298162/P1_19_mod.zip) All commands used are below **last-train:** ``` cd termitodb lastdb -uNEAR -R01 termitodb ../Termitomyces_v3.0.fa ```...

I am using a job submission system yes. This is all running in slurm. What would the job submission system be doing to cause this error? I can try run...