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Hi @itslittman, received the sam and can confirm that it's possible to fix the offending lines with the following: `sed -E 's/ [0-9]+->[0-9]+$//g' split.head.sam > split.fixed.sam` Can you let me...

Hi @Coryza, you would be better off relying on the read splitting that is already integrated in guppy, it works in a similar fashion to split_on_adapter and should make for...

Hi Koen, Sorry for later reply this time. There is a .cfg file in the barcoding folder in guppy/data where you can change the mid-adapter. I would have to refer...

Thanks @GeoMicroSoares, just checking internally whether pyfastx can be either pinned to a previous version or whether the code can be updated in ont_guppy_duplex_pipeline! In the meantime, can you try...