PietroD

Subsetted to a thousand cells but got the same error

[5435796.log](https://github.com/user-attachments/files/21776455/5435796.log) ``` host: srcn02 Building DAG of jobs... Using shell: /usr/bin/bash Provided remote nodes: 1 Provided resources: mem_mb=8000, mem_mib=7630, disk_mb=36578, disk_mib=34884, gpu=0 Select jobs to execute... Execute 1 jobs... [Fri...

Thanks @quentinblampey Yes the log is correct (I changed some paths but it is correct). Unfortunately this error occurred to me already a couple of times. Could it be related...

No I was running one sample only, but several algorithms on the same sample, don’t know if this is relevant. Yes Nextflow implementation of ComSeg would be great! We have...

@quentinblampey You are a productivity machine! Thanks a lot!! I'll let you know as soon as I try it

Thanks @ajitjohnson ! You are right indeed, in my case the coordinates are in microns, and not starting from 0: ``` spatial_X spatial_Y min max min max ParentID a 8978.1...

Thanks @ajitjohnson ! Indeed, dividing by the resolution factor (0.5 μm/pixel) puts the image and the gates in the "almost" same scale. As you can see, there is still a...