Pentayouth

because the gene you used to define cel type in cth( ) failed to be one of the "expressed_genes"

add my experience here for those feeling anxious like me. 20000 genes * 20000 cells 30 core 9hr30min

normalizedata slow findvariable feature slow scaledata slow runpca slow sketch data slow (run sketchdata before i sleep and find it stilling running after i wake up.) everything get slow after...

New doubletfinder only works for those Seurat V5 objects without BPCell preprocess

i encountered the same issue. however only when integrating >50 samples / 300K cells. i have to wait for 20min before joinlayer finished join 80 samples. which is crazy. i...

I have two guesses: 1. the problem caused by call bpcell on each sample separately, so matrix were created into different folders. 2. genes between samples dont match perfectly.

> > add my experience here for those feeling anxious like me. > > 20000 genes * 20000 cells 30 core > > 9hr30min > > Thanks for sharing! @Pentayouth...

> > > What worked for me was to rename the colnames of the GRNBoost output .tsv required by the downstream wrapper runSCENIC_1_coexNetwork2modules() and save the file in the respective...