premessa
premessa copied to clipboard
ParkerICI
R package for pre-processing of mass and flow cytometry data
Hi, I had an issue with the beads in the first batch of my mass cytometry experiment. The beads were sticking to the plastic tube and therefore not enough were...
Hi! I'm reading many fcs files at the same time. Is there anyway I can save/download the paneleditor_GUI() results as a csv?
It would be nice if there were an option to select Custom channels for beads. This was a feature in the original Finck MATLAB normalizer ("Custom"). This would help address...
Hello, I viewed my files in FlowJo pre- and post-Premessa panel editing and noticed a discrepancy. Each marker's plot got altered post-panel editing. Below are the plots for the same...
lazy-load database '/Library/Frameworks/R.framework/Versions/4.2-arm64/Resources/library/premessa/R/premessa.rdb' is corrupt  > sessionInfo() R version 4.2.2 (2022-10-31) Platform: aarch64-apple-darwin20 (64-bit) Running under: macOS Ventura 13.2.1 Matrix products: default LAPACK: /Library/Frameworks/R.framework/Versions/4.2-arm64/Resources/lib/libRlapack.dylib locale: [1] en_US.UTF-8/en_US.UTF-8/en_US.UTF-8/C/en_US.UTF-8/en_US.UTF-8 attached base...
Hi there, Using premessa panel editor and selecting a file in a folder containing "PATHWITHDRIVE/FILENAME.FCS" files, we had the issue that no files were found by premessa, which resulted in...
Hey, I'm trying to debarcode a file that have been barcoded with a 7 choose 3 scheme. When debarcoding, the package debarcode based on the combinations that have been described...
Hello, I hope you're well. Lately, when I have been using the premessa Debarcoder, the cell counts I get on the y-axis of the bar chart and in the separation...
Hi, Is there an explicit order of operations for the steps of Premessa? From the order in the Wiki, my assumption is that concatenation should happen before normalization. And that's...
Fluidigm has launched new normalizer beads with 6 elements: "Description: EQ™ Six Element Calibration Beads (EQ6 beads) is a 1X stock solution (used at 0.1X for sample acquisition) of polystyrene...