paneleditor_GUI() $PnR value truncated

[lchan8]

Hello,

I viewed my files in FlowJo pre- and post-Premessa panel editing and noticed a discrepancy. Each marker's plot got altered post-panel editing. Below are the plots for the same sample, before and after panel editing. The channels are correctly renamed.

I noticed that I am getting the following warnings when I run paneleditor_GUI(). How do I fix $PnR? Warning in readFCSdata(con, offsets, txt, transformation, which.lines, scale, : Some data values of 'Yb171Di' channel exceed its $PnR value 1 and will be truncated! To avoid truncation, either fix $PnR before generating FCS or set 'truncate_max_range = FALSE' Warning in readFCSdata(con, offsets, txt, transformation, which.lines, scale, : Some data values of 'beadDist' channel exceed its $PnR value 1 and will be truncated! To avoid truncation, either fix $PnR before generating FCS or set 'truncate_max_range = FALSE'

With paneleditor_GUI(), there is no option to set 'truncate_max_range = FALSE'. My flowCore version is ‘2.10.0’