Primer Trimming Optimization

[atfdh]

Hi, I noticed that when I do not use the --skip_primer_trim option in the command, the number of input reads decreases significantly as demuxed reads, sometimes by as much as 7 times. why does it happen? how I can remove primers without losing a high number of reads?

[proteinosome]

@atfdh By any chance the primers are already removed in your reads? I'm assuming these are standard F27 and R1492 primers for full-length 16S?

[atfdh]

Thanks, I understood and solved the problem!