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feature request to feed FALCON single FASTA

Open kevfengler227 opened this issue 7 years ago • 1 comments

I’d like to put in a feature request to have FALCON accept a single FASTA in the input.fofn. This would be lot more convenient in many ways, particularly for large genome projects where the filtered subreads for all cells could be combined into one file. Also, for targeted assembly projects I’d like to be able to feed FALCON a subset of reads that were identified by alignment to a target sequence and not have to split it up again by cell.

Thanks, KF

kevfengler227 avatar Jul 22 '16 20:07 kevfengler227

The restriction is in Gene Myers' fasta2DB. However, that was made more flexible recently. We'll have to do some testing to see how it works today.

If you have a problem with the latest code, look for fasta2fasta.py, which can be used to split one into several. That was briefly part of our workflow. With that work-around, this is relatively low priority, but I will look into it when I have a chance.

pb-cdunn avatar Jul 24 '16 15:07 pb-cdunn