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add adapters to known adapters file

Open CatarinaCarolina opened this issue 7 months ago • 1 comments

Hi all,

I just received Illumina NovaSeq PE sequencing data, and according to the sequencing company, the following adapters were used:

Sequences of adapter

P5 adapter:

P5→P7’(5’→3’)

AATGATACGGCGACCACCGAGATCTACAC[i5*]ACACTCTTTCCCTACACGACGCTCTTCCGATCT

P7 adapter:

P5→P7’(5’→3’)

GATCGGAAGAGCACACGTCTGAACTCCAGTCAC[i7*]ATCTCGTATGCCGTCTTCTGCTTG

*i5/i7 represent index sequences. They are provided here to show the complete adapter structure. Requirements for including or excluding indices in adapter trimming software may vary.

I can find fragments, but not the entirety of the adapters, in the https://github.com/OpenGene/fastp/blob/master/src/knownadapters.h file. Could you check if these need adding, and if so, add them?

Kindly, Catarina

CatarinaCarolina avatar Jun 11 '25 14:06 CatarinaCarolina

can you run the new fastp to check whether fastp can detect the adapter correctly?

sfchen avatar Jul 02 '25 06:07 sfchen