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add adapters to known adapters file
Hi all,
I just received Illumina NovaSeq PE sequencing data, and according to the sequencing company, the following adapters were used:
Sequences of adapter
P5 adapter:
P5→P7’(5’→3’)
AATGATACGGCGACCACCGAGATCTACAC[i5*]ACACTCTTTCCCTACACGACGCTCTTCCGATCT
P7 adapter:
P5→P7’(5’→3’)
GATCGGAAGAGCACACGTCTGAACTCCAGTCAC[i7*]ATCTCGTATGCCGTCTTCTGCTTG
*i5/i7 represent index sequences. They are provided here to show the complete adapter structure. Requirements for including or excluding indices in adapter trimming software may vary.
I can find fragments, but not the entirety of the adapters, in the https://github.com/OpenGene/fastp/blob/master/src/knownadapters.h file. Could you check if these need adding, and if so, add them?
Kindly, Catarina
can you run the new fastp to check whether fastp can detect the adapter correctly?