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Bug when deal with phred 64 reads
Hi,
I found if I use fastp to process phred 64 reads (with -6
), if the data amount is large (such as >20,000 reads, haven't tested the exact number),
- fastp cannot correct calculate the Q20/Q30 base and the quality_curves is still >60 (haven't changed it to phred 33 to show).
- the result cannot be repeated (the results were slightly different each time with the same parameters).
However, if the data amount is small (say <15000), everything is fine.
This situation only happens in the version > 0.20.0. In v 0.20.0, everything is fine (both the read quality and result between different runs).
I am not sure whether it is related the speed up after 0.20.0?
Many thanks, Raymond