AfterQC total bases calculation

[pbravakos]

AfterQC is constantly counting wrong the total bases in paired end Miseq fastq reads. For example two paired end files with total bases 611,060,153 (as calculated by various programs) it seems to have only 582,146,000 total bases in AfterQC. I was wondering why this difference exists, and if it affects the downstream filtering process.

[sfchen]

AfterQC treats 1024 as 1K, while others treat 1000 as 1K, that's the difference.

I will revise AfterQC to treat 1000 as 1K.

BTW, I suggest you to use fastp instead of AfterQC. fastp is much better than AfterQC, and 10X faster.

See https://github.com/OpenGene/fastp