LiuMin

Hello,sorry to bother you. I have the same problem, have you solved it?

I‘m so glad to get your reply, I would like to ask is it reasonable to split the reference like this? Will it affect the result of mapping?

ok, thank you,you are so nice.

hi, heng. I'm glad to receive your reply, because I don't have so much memory to deal with large ref. I want to split large reference genomes like minimap2, divide...

> Hey, I'm having the same kind of problem. > > I aligned my PE reads against the transcriptome using BWA-mem and then sorted them by coordinates (as a regular...

Hi, @shilpagarg @iromalekou ,I have the same problem. Can i ignore these warnings? 

Salmon detected suspicious pair, will it be quantified?

Thanks for your quick reply. My bam file is filtered, and there will be some single-ended reads. I don’t know how to deal with these 'orphan'. The program give me...

Thanks for your quick reply. My bam files were genrated by BWA. If I want to take into account the read of this single-end alignment, I need to add its...

ok, I get it. Thanks a millon.🌼