LiuMin

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Hello,sorry to bother you. I have the same problem, have you solved it?

I‘m so glad to get your reply, I would like to ask is it reasonable to split the reference like this? Will it affect the result of mapping?

hi, heng. I'm glad to receive your reply, because I don't have so much memory to deal with large ref. I want to split large reference genomes like minimap2, divide...

> Hey, I'm having the same kind of problem. > > I aligned my PE reads against the transcriptome using BWA-mem and then sorted them by coordinates (as a regular...