Medhat

Can you please write down how did you run it?

I am assuming you run it from the directory you installed princess in. so make sure it exists `ls princess ` and lastly try './princess'

I see, you need to make slight change after `cd princess` run it like `./princess -h`

OK, first now you know why it was showing this message "command not found". We have another error now, please copy and paste it here.

OK, this is more an Ubuntu issue, based on this answer: https://stackoverflow.com/questions/56935698/attributeerror-nonetype-object-has-no-attribute-loader

Any news about this update?

You need to install samtools first. E.g., `conda install samtools` if you have Conda installed. For more details regarding samtools installation; please, visit http://www.htslib.org/download/

> thanks @MeHelmy! I installed samtools successfully but now there is a new error besides that, I still can´t this line (princess all -d ./princess_all -r ont -s reads.split00.fastq.gz reads.split01.fastq.gz...

Sorry for the late answer, Which conda version you are using?

Hi, we just made an update to reduce Conda dependency. I hope that you can give it a try.